SDS-Page Solutions

Lower Gel Buffer (LGB 4x)

• 90.9 g. 1.5 M Tris
• 2 g. 0.4% SDS
• Adjust pH to 8.8
• q.s. to 500 mL w/ ddH₂O

Upper Gel Buffer (UGB 4x)

• 30.3 g. 0.5 M Tris
• 2 g. 0.4% SDS
• Adjust pH to 6.8
• q.s. to 500 mL w/ ddH₂O

30% Acrylamide

• 150 g. 29.2% Acrylamide
• 4.1 g. 0.8% Bis-Acrylamide
• q.s. to 513.5 mL w/ ddH₂O

Filter sterilize; store at 4^oC; store in opaque/dark bottle.