Chromatography Facility

Dr. Jiping Zou (Director)
S-125 ESC
(801) 422-4075
jiping_zou@byu.edu



 


The College of Life Sciences has established a Chromatography Facility for the use of researchers who do not have access to or expertise in chromatographic analyses. The Facility Director is Dr. Jiping Zou. Anyone interested in using the facility can contact Dr. Zou to schedule a meeting.

Capabilities


Chromatographic Capabilities

  • HPLC and LC-MSD (6 instruments)
  • GC and GC-MSD (4 instruments)
  • Headspace sampler for GC (1 instrument)
  • Detectors (DAD, FLD, RI, FID, ECD, MSD,TCD)
  • SPE extraction
  • GERSTEL MPS system for GC SPME and Headspace sampling technology
  • Thermostat controlled auto-sampler system
  • Preparative sample fractionation and isolation using HPLC or open column chromatography

Examples of Analyses Available

  • Alkaloids (HPLC, GC)
  • Amino acids (HPLC)
  • Carbohydrates (GC)
  • Fatty acids (GC)
  • Flavanoids (HPLC)
  • Isoflavones (HPLC)
  • Organic acids (HPLC)
  • Sterols (HPLC)
  • Terpenes (HPLC, GC)
  • Vitamins B1, B2, C, A, D, E (HPLC, GC)
  • Nucleotides (HPLC)
  • Protein, Peptide and Amino acid (HPLC)
  • Glycinebetaine (HPLC)

General Policies and Protocol


The Facility analyzes samples prepared by the Researcher; i.e., extractions and sample preparations are done by the Researcher, before taken to the Facility. The extract must be filtered with at least 0.45 m pore size filter; the analyte should be at least 2~5 g/ml for UV, MSD and FID detectors, and 0.1 g/ml for fluorescence detector.

The facility has the columns necessary for most applications, at no additional cost. If a specific column is needed and the facility does not have the column, the researcher will be required to purchase the necessary column. Researchers and students may observe, but may not perform analyses If method development or sample extraction is needed, costs will be determined in consultation with Director. The development of policies and procedures is ongoing, under the supervision of the Chromatography Facility Committee, as is the review of these procedures by Users.

Procedure to Activate a Project

  1. Researcher and Director meet to discuss project, including:
    1. Methods to be used; method development, if necessary
    2. Sample preparation needs
    3. Column needs
    4. Type of data to be generated costs; how costs are determined, when payment is due, and billing procedure
    5.  When the analyses should be completed
  2. Notes on discussion are provided to the User and the Facility Director using duplicated forms
  3. User account is set up

Costs


Rates are subject to change, based on method required.

Test Samples Price/Sample
HPLC Analysis 1 - 10 $10
11 + $8
GC Analysis 1 - 10 $10

11 + $8
Sample Preparation 1 - 10 $40

11 - 50 $30

51 - 100 $25

101 + $20

Initial set up fee is $50 for each new project (i.e., new method) that is requested. GC - MS and HPLC-MS compound identification costs $15 per sample. Compound isolation and purification ~$300 - $500, depending on procedure.

Some factors affecting analysis costs:

  1. Instrument maintenance (Including service contracts)
  2. Student labor
  3. Consumable supplies (solvents, vials, etc.)
  4. Columns provided by User generally (resolved at consultation)
  5. Number of samples
  6. Method development
  7. Consultation time



Last Updated: August 13, 2009